Journal: Molecular Medicine Reports

Article Title: Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis

doi: 10.3892/mmr.2016.5584

Figure Lengend Snippet: Expression levels of CD1, CD133, CK18, α-SMA and vimentin indicated by immunohistochemical staining. Expression levels of (A) CD1, (B) CD133, (C) CK18, (D) α-SMA and (E) vimentin, in samples no. 1, 2 and 3 (left to right). CD1, cyclin D1; CD133, cluster of differentiation 133; CK18, cytokeratin 18; α-SMA, α-smooth muscle actin.

Article Snippet: The primary antibodies used in the IHC staining in the present study were as follows: Rabbit polyclonal against E-cadherin (Abcam, Cambridge, MA, USA; cat. no. ab15148), rat monoclonal against CRB3 (Abcam; cat. no. ab180835), rabbit monoclonal against α-SMA (Abcam; cat. no. 124964), rabbit polyclonal against PAR-3 (Bioss, Inc., Woburn, MA, USA; cat. no. bs-9510R), rabbit polyclonal against Hyal-1 (Abcam; cat. no. ab103977), mouse monoclonal against Col-I (Abcam; cat. no. ab6308), rabbit polyclonal against cytokeratin 18 (CK18; Bioss, Inc.; cat. no. bs-1339R), rabbit polyclonal against vimentin (Bioss, Inc.; cat. no. bs-8533R), rabbit polyclonal against CD1 (Bioss, Inc.; cat. no. bs-0623R) and rabbit polyclonal against CD133 (Bioss, Inc.; cat. no. bs-0395R).

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Molecular Medicine Reports

Article Title: Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis

doi: 10.3892/mmr.2016.5584

Figure Lengend Snippet: CCA value of ten biomarkers in samples No. 1, No. 2 and No. 3. Demonstrated by the CCA value, the expression levels of E-cadherin, CK18, Crumbs-3 and Par-3 in sample No. 3 were lower compared with sample No. 1 (P<0.05). The expression of CK18 in sample No. 2 was lower than in sample No. 3 (P<0.0001). The expression levels of Hyal-1, Col-I, vimentin, CD1, CD133 and α-SMA in sample No. 3 were higher than in sample No. 1 (P<0.05), however, the expression levels of Hyal-1, Col-I, α-SMA in sample No. 3 were lower than that in sample No. 2, the result for Hyal-1 was not significant. CCA, corrected absorbance value; CK18, cytokeratin 18; Par-3, proteinase activated receptor 3; Hyal-1, hyaluronidase 1; Col-I, collagen type I; CD1, cyclin D1; CD133, cluster of differentiation 133; α-SMA, α-smooth muscle actin.

Article Snippet: The primary antibodies used in the IHC staining in the present study were as follows: Rabbit polyclonal against E-cadherin (Abcam, Cambridge, MA, USA; cat. no. ab15148), rat monoclonal against CRB3 (Abcam; cat. no. ab180835), rabbit monoclonal against α-SMA (Abcam; cat. no. 124964), rabbit polyclonal against PAR-3 (Bioss, Inc., Woburn, MA, USA; cat. no. bs-9510R), rabbit polyclonal against Hyal-1 (Abcam; cat. no. ab103977), mouse monoclonal against Col-I (Abcam; cat. no. ab6308), rabbit polyclonal against cytokeratin 18 (CK18; Bioss, Inc.; cat. no. bs-1339R), rabbit polyclonal against vimentin (Bioss, Inc.; cat. no. bs-8533R), rabbit polyclonal against CD1 (Bioss, Inc.; cat. no. bs-0623R) and rabbit polyclonal against CD133 (Bioss, Inc.; cat. no. bs-0395R).

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis

doi: 10.3892/mmr.2016.5584

Figure Lengend Snippet: Heatmap of biomarker expression in the present study according to the CCA value of samples No. 1, No. 2 and No. 3. The heatmap diagram indicates the result of the two-way hierarchical clustering of markers and samples. Each row represents a marker and each column represents a sample. The marker clustering tree is presented on the right, and the sample clustering tree at the top. Cluster analysis arranges samples and markers into groups based on their CCA values, which allows formation of hypotheses regarding the association between markers and samples. The color of each pattern indicates the CCA value, green to red is −0.8 to 1.0. CCA, corrected absorbance value; α-SMA, α-smooth muscle actin; CD1, cyclin D1; CD133, cluster of differentiation 133; PAR-3, proteinase activated receptor 3; Col-I, collagen type I; Hyal-1, hyaluronidase 1.

Article Snippet: The primary antibodies used in the IHC staining in the present study were as follows: Rabbit polyclonal against E-cadherin (Abcam, Cambridge, MA, USA; cat. no. ab15148), rat monoclonal against CRB3 (Abcam; cat. no. ab180835), rabbit monoclonal against α-SMA (Abcam; cat. no. 124964), rabbit polyclonal against PAR-3 (Bioss, Inc., Woburn, MA, USA; cat. no. bs-9510R), rabbit polyclonal against Hyal-1 (Abcam; cat. no. ab103977), mouse monoclonal against Col-I (Abcam; cat. no. ab6308), rabbit polyclonal against cytokeratin 18 (CK18; Bioss, Inc.; cat. no. bs-1339R), rabbit polyclonal against vimentin (Bioss, Inc.; cat. no. bs-8533R), rabbit polyclonal against CD1 (Bioss, Inc.; cat. no. bs-0623R) and rabbit polyclonal against CD133 (Bioss, Inc.; cat. no. bs-0395R).

Techniques: Biomarker Assay, Expressing, Marker

Journal: PLoS ONE

Article Title: Endothelial cells are a source of Nestin expression in Pulmonary Arterial Hypertension

doi: 10.1371/journal.pone.0213890

Figure Lengend Snippet: ( A ) Representative flow cytometry of rat lung ECs for CD144 (Vascular Endothelial-cadherin) and VEGFR2. Rat lung ECs were negative for myeloid/hematopoietic markers CD133 and CD11b/c. The specific antibody staining is red, and the corresponding isotype is grey. ( B ) Rat lung ECs bind Griffonia simplicifolia lectin (G.s.), indicating microvascular ECs. ( C ) Rat lung ECs grown on chamber slides express Nestin. Note the perinuclear localization and the filaments extending throughout the cytoplasm. Control means omission of primary antibody. (B-C): Scale bars: 50 μm. ( D ) Representative Western blot showing Nestin expression in HLMVECs (β-actin as loading control). ( E ) Representative Western blots showing PCNA expression in HLMVECs (α-tubulin as loading control). Experiments 1–3 indicate unstimulated cells grown in separate dishes in EGM-2MV for Western blot analysis.

Article Snippet: Expression of myeloid/hematopoietic markers CD133 (bs-0209R, Bioss) and CD11b/c (554862, BD Biosciences) was excluded by flow cytometry.

Techniques: Flow Cytometry, Staining, Western Blot, Expressing

Journal: Stem Cell Research & Therapy

Article Title: The effect of endothelial progenitor cell transplantation on neointimal hyperplasia and reendothelialisation after balloon catheter injury in rat carotid arteries

doi: 10.1186/s13287-021-02135-w

Figure Lengend Snippet: Identification of bone marrow-derived EPCs. a Adherent cells formed a cluster on the fifth day of culture. b The cell cluster grew into a colony on the seventh day of culture. c The typical colonies, consisting of a central core of rounded cells surrounded by spindle-shaped cells, appeared at the second day post-passage. Scale bar = 100 μm ( a – c ). Cells positive for FITC-UEA-1 staining ( d , green) and Dil-ac-LDL staining ( e , red). f Merged images represented double-positive for the uptake of Dil-ac-LDL and binding with FITC-UEA-1. The double-positive cells accounted for 85% of the total. Confocal microscopic images ( g , red), bright-field microscopic images ( h ), and merged images of PKH26-labelled bone marrow-derived EPCs ( i ). Scale bar = 50 μm ( d – i ). j Cells were positive for CD34 in flow cytometry. k Cells were positive for CD34, VEGFR2, and CD133. The triple-positive cells accounted for 75% of the total

Article Snippet: After resuspension in fluorescence-activated cell sorting buffer (100 μL), 5 × 10 5 cells were incubated with rabbit anti-CD133/FITC conjugated antibody (1:100; Bioss Co., Ltd., Beijing, China, bs-4770R-FITC), CD34 monoclonal antibody (QBEND/10), PE (1:10; Thermo Fisher Scientific Inc., MA, USA, MA1-10205), and VEGFR2 (D5B1) rabbit mAb (Alexa Fluor® 647 conjugate) (1:50; Cell Signaling Technology Inc., MA, USA, 12658) for 60 min at 4 °C in the dark.

Techniques: Derivative Assay, Staining, Binding Assay, Flow Cytometry

Journal: Journal of Hepatocellular Carcinoma

Article Title: Re-Recognizing the Cellular Origin of the Primary Epithelial Tumors of the Liver

doi: 10.2147/JHC.S334935

Figure Lengend Snippet: Monoclonal Antibodies Used in This Study

Article Snippet: Rabbit anti-human CD133 , 1:100 , EMK08 , OriGene Tech. , Rockville, MA, USA , TA350943.

Techniques: